Isabella is the young wife of Alibius. She is attractive to men and her old husband fears that her affections may stray. Confined to a room where she may only meet the inmates of the lunatic asylum rather than the visitors, she finds herself subject to the unwanted romantic attentions of Antonio and Franciscus. She also has to fend off an attempted seduction by Lollio. Isabella's common sense and good judgment are contrasted with Beatrice's complete lack of those qualities.

Isabella means "God's Promise" and Isabella does keep her promise to her husband.

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